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Present hypotheses indicate that a testis differentiation cascade in mammals is induced by Sry, a gene encoding a DNA binding protein of the high mobility group (HMG) class.

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In most mammals, sex determination is controlled genetically by the presence or absence of SRYSry (sex-determining region on the Y chromosome), which encodes a putative transcription factor containing a high-mobility group-box DNA-binding domain (Gubbay et al., 1990; Sinclair et al., 1990; Koopman et al., 1991).

Although no ortholog for SRY has been identified in non-mammalian vertebrates, a Y chromosome-linked gene, DMY (DM domain gene on the Y chromosome, also known as dmrt1b Y), was found to be a prime candidate for the sex-determining gene in the teleost medaka (Matsuda et al., 2002; Nanda et al., 2002).

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